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njlpkhgea
Wysłany: Śro 15:43, 02 Mar 2011
Temat postu: Acute leukemia cells in vitro on Jurkat cell apopt
,
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Acute leukemia cells in vitro apoptosis of Jurkat cells
(P <0.05), both of which increased with cell concentration increased apoptosis. ANII group plus anti FasI with and without anti-apoptotic rate was significantly reduced compared FasI (P <0.01), with anti FasI reduced apoptosis rate increase with increasing concentration; AII group plus anti FasI compared with and without anti-apoptotic FasI rate slightly decreased (P <0.05), but also with the anti-apoptotic FasI reduce the rate of increase with increasing concentration, but not as good as ANII apoptosis rate changed significantly (see Table 1). Table 1Jurkat cells incubated with different concentrations of the apoptosis of leukemia cells compare to the rates (± 5.) Note: Compared with control group. P <0.01, P <0.05, ANLL group were compared with and without anti-FasL ~ P <0.01, ALL group were compared with and without anti-Fasl P <0.053 FaS discussed in combination with FasL the only way to play a role in the body. Initially thought, FasL only in lymphoid and myeloid cells, the expression, recent studies have shown that some non-lymphoid tumors express FasL [. FasI start of apoptosis is an important factor, the surface of target cells with Fas cross-linking, may induce apoptosis r7]. Anti-Fas antibodies, cell surface or plasma sFasI FasI can be combined with cell surface Fas, leading to cell apoptosis]. The study found, ANLL can cause increased apoptosis Jurkat cells, indicating that FasL expression ANLL cells, after adding anti FasI, Jurkat cell apoptosis was significantly reduced, indicating the existence of leukemia cells in the functional expression of FasL. AII induced only a mild increase in apoptotic Jurkat cells, indicating the expression of AIA cells FasI weaker than ANII, which may be part of AIL in the expression of Fas on T lymphocytes, induced by anti FasI of Jurkat cells also decreased, but the cell plus the same concentration of high concentration of anti FasI, AII level changes in apoptotic cells was not as good as ANII, which may be different between the expression of FasL on the level, the mechanism needs further study. Our results show that the expression of leukemia cells by immune cells FasI to Fas apoptosis pathway, and thus evade the immune system's surveillance for tumor immunotherapy that provides a new idea. 4
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