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 Wysłany: Pon 13:00, 07 Mar 2011 Temat postu: nike sale Anti-human heterogeneous nuclear ribonuc |
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Anti-human heterogeneous nuclear ribonucleoprotein A2/B1 antibody variable region gene, tandem and expression
ElISA, Western blot and immunohistochemistry experiments not only confirmed that 3E8 antibody specifically binding with HnRNPA2/B1 synthetic peptides,[link widoczny dla zalogowanych], but also the natural HnRNPA2/B1 intracellular protein binding,[link widoczny dla zalogowanych], and a lung cancer cell lines YTMLC 90 and A2 are also high Expression HnRNPA2/B1. On this basis, we used reverse transcription PCR cloned from the hybridoma cell line VH and VI genes, and through SOE-PCR successfully VH and VL series into ScFv genes,[link widoczny dla zalogowanych], the recombinant expression vector pET28 a ScFv, and Escherichia coli obtained in the functional expression of genes for further reform of the ScFv construct antibodies against HnRNPA2/B1 cell research foundation.
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